Controllable Acoustofluidic Rotation and Three-Dimensional Reconstruction of Single Cells

Traditional live single-cell 3D imaging techniques face challenges such as photobleaching, invasive labeling, and mechanical rotation limitations, which hinder long-term, high-precision observation of cellular dynamics. To address these issues, this paper proposes an integrated platform for non-contact rotation and microscopic imaging based on acoustofluidic actuation, along with a 3D cell reconstruction method based on the optical flow algorithm. The platform employs a piezoelectric transducer to induce microbubble resonance, which generates programmable fluid vortices to drive cells in rotation along multiple axes, solving the problems of rotational instability and cell damage. Additionally, this study introduces a 3D cell reconstruction algorithm based on the optical flow method, which tracks the motion trajectories of surface feature points on the cell, extracts rotational angle information without fluorescent labeling, and reconstructs the cell contour. This technology offers an innovative solution for live-cell 3D imaging, overcoming several limitations of traditional methods while demonstrating advantages in long-term, high-precision observations.